part1
Doxycycline (doxycycline hyclate, doxycycline hydrochloride or Dox), a synthetic tetracycline (Tc) derivative, is the effector molecule for Clontech's Tet-On® and Tet-Off® Inducible Expression Systems. Dox can be used at 100-fold lower concentrations than Tc, yielding inducing concentrations ranging from 1ng/ml to 1 µg/ml. In the Tet-On System, addition of Dox to culture medium induces a conformational change in the transactivator which allows it to bind to tet operator sequences located in the inducible promoter. This results in transcriptional activation of the gene of interest. In the Tet-Off System, removal of Dox from culture medium induces binding of the transactivator, resulting in transcription of the gene of interest.
part2
Doxycycline (doxycycline hyclate, doxycycline hydrochloride or Dox), a synthetic tetracycline (Tc) derivative, is the effector molecule for Clontech's Tet-On® and Tet-Off® Inducible Expression Systems. Dox can be used at 100-fold lower concentrations than Tc, yielding inducing concentrations ranging from 1ng/ml to 1 µg/ml. In the Tet-On System, addition of Dox to culture medium induces a conformational change in the transactivator which allows it to bind to tet operator sequences located in the inducible promoter. This results in transcriptional activation of the gene of interest. In the Tet-Off System, removal of Dox from culture medium induces binding of the transactivator, resulting in transcription of the gene of interest.
part3
Doxycycline (doxycycline hyclate, doxycycline hydrochloride or Dox), a synthetic tetracycline (Tc) derivative, is the effector molecule for Clontech's Tet-On® and Tet-Off® Inducible Expression Systems. Dox can be used at 100-fold lower concentrations than Tc, yielding inducing concentrations ranging from 1ng/ml to 1 µg/ml. In the Tet-On System, addition of Dox to culture medium induces a conformational change in the transactivator which allows it to bind to tet operator sequences located in the inducible promoter. This results in transcriptional activation of the gene of interest. In the Tet-Off System, removal of Dox from culture medium induces binding of the transactivator, resulting in transcription of the gene of interest.
part4
Doxycycline (doxycycline hyclate, doxycycline hydrochloride or Dox), a synthetic tetracycline (Tc) derivative, is the effector molecule for Clontech's Tet-On® and Tet-Off® Inducible Expression Systems. Dox can be used at 100-fold lower concentrations than Tc, yielding inducing concentrations ranging from 1ng/ml to 1 µg/ml. In the Tet-On System, addition of Dox to culture medium induces a conformational change in the transactivator which allows it to bind to tet operator sequences located in the inducible promoter. This results in transcriptional activation of the gene of interest. In the Tet-Off System, removal of Dox from culture medium induces binding of the transactivator, resulting in transcription of the gene of interest.